A novel reporter system for neutralizing and enhancing antibody assay against dengue virus
Identifieur interne : 000A51 ( Main/Exploration ); précédent : 000A50; suivant : 000A52A novel reporter system for neutralizing and enhancing antibody assay against dengue virus
Auteurs : Ke-Yu Song [République populaire de Chine] ; Hui Zhao [République populaire de Chine] ; Zhen-You Jiang [République populaire de Chine] ; Xiao-Feng Li [République populaire de Chine] ; Yong-Qiang Deng [République populaire de Chine] ; Tao Jiang [République populaire de Chine] ; Shun-Ya Zhu [République populaire de Chine] ; Pei-Yong Shi [Singapour] ; Bo Zhang [République populaire de Chine] ; Fu-Chun Zhang [République populaire de Chine] ; E-De Qin [République populaire de Chine] ; Cheng-Feng Qin [République populaire de Chine]Source :
- BMC Microbiology [ 1471-2180 ] ; 2014.
Abstract
Dengue virus (DENV) still poses a global public health threat, and no vaccine or antiviral therapy is currently available. Antibody plays distinct roles in controlling DENV infections. Neutralizing antibody is protective against DENV infection, whereas sub-neutralizing concentration of antibody can increase DENV infection, termed antibody-dependent enhancement (ADE). Plaque-based assay represents the most widely accepted method measuring neutralizing or enhancing antibodies.
In this study, a novel reporter virus-based system was developed for measuring neutralization and ADE activity. A stable
This reporter virus-based assay for neutralizing and enhancing antibody evaluation is rapid, lower cost, and high throughput, and will be helpful for laboratory detection and epidemiological investigation for DENV antibodies.
Url:
DOI: 10.1186/1471-2180-14-44
PubMed: 24548533
PubMed Central: 3930823
Affiliations:
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Jiang</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhu, Shun Ya" sort="Zhu, Shun Ya" uniqKey="Zhu S" first="Shun-Ya" last="Zhu">Shun-Ya Zhu</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Shi, Pei Yong" sort="Shi, Pei Yong" uniqKey="Shi P" first="Pei-Yong" last="Shi">Pei-Yong Shi</name><affiliation wicri:level="1"><nlm:aff id="I3">Novartis Institute for Tropical Diseases, Singapore 138670, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Novartis Institute for Tropical Diseases, Singapore 138670</wicri:regionArea><wicri:noRegion>Singapore 138670</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Bo" sort="Zhang, Bo" uniqKey="Zhang B" first="Bo" last="Zhang">Bo Zhang</name><affiliation wicri:level="1"><nlm:aff id="I4">State Key Laboratory of Virology, Wuhan Institute of Virology, CAS, Wuhan 430072, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Virology, Wuhan Institute of Virology, CAS, Wuhan 430072</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zhang, Fu Chun" sort="Zhang, Fu Chun" uniqKey="Zhang F" first="Fu-Chun" last="Zhang">Fu-Chun Zhang</name><affiliation wicri:level="1"><nlm:aff id="I5">Guangzhou No.8 People’s Hospital, Guangzhou 510060, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangzhou No.8 People’s Hospital, Guangzhou 510060</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Qin, E De" sort="Qin, E De" uniqKey="Qin E" first="E-De" last="Qin">E-De Qin</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 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wicri:level="1"><nlm:aff id="I2">Department of Microbiology and Immunology, School of Medicine Jinan University, Guangzhou 510632, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Medicine Jinan University, Guangzhou 510632</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhao, Hui" sort="Zhao, Hui" uniqKey="Zhao H" first="Hui" last="Zhao">Hui Zhao</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Jiang, Zhen You" sort="Jiang, Zhen You" uniqKey="Jiang Z" first="Zhen-You" last="Jiang">Zhen-You Jiang</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Microbiology and Immunology, School of Medicine Jinan University, Guangzhou 510632, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Medicine Jinan University, Guangzhou 510632</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Li, Xiao Feng" sort="Li, Xiao Feng" uniqKey="Li X" first="Xiao-Feng" last="Li">Xiao-Feng Li</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Deng, Yong Qiang" sort="Deng, Yong Qiang" uniqKey="Deng Y" first="Yong-Qiang" last="Deng">Yong-Qiang Deng</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Jiang, Tao" sort="Jiang, Tao" uniqKey="Jiang T" first="Tao" last="Jiang">Tao Jiang</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhu, Shun Ya" sort="Zhu, Shun Ya" uniqKey="Zhu S" first="Shun-Ya" last="Zhu">Shun-Ya Zhu</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Shi, Pei Yong" sort="Shi, Pei Yong" uniqKey="Shi P" first="Pei-Yong" last="Shi">Pei-Yong Shi</name><affiliation wicri:level="1"><nlm:aff id="I3">Novartis Institute for Tropical Diseases, Singapore 138670, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Novartis Institute for Tropical Diseases, Singapore 138670</wicri:regionArea><wicri:noRegion>Singapore 138670</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Bo" sort="Zhang, Bo" uniqKey="Zhang B" first="Bo" last="Zhang">Bo Zhang</name><affiliation wicri:level="1"><nlm:aff id="I4">State Key Laboratory of Virology, Wuhan Institute of Virology, CAS, Wuhan 430072, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Virology, Wuhan Institute of Virology, CAS, Wuhan 430072</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zhang, Fu Chun" sort="Zhang, Fu Chun" uniqKey="Zhang F" first="Fu-Chun" last="Zhang">Fu-Chun Zhang</name><affiliation wicri:level="1"><nlm:aff id="I5">Guangzhou No.8 People’s Hospital, Guangzhou 510060, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangzhou No.8 People’s Hospital, Guangzhou 510060</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Qin, E De" sort="Qin, E De" uniqKey="Qin E" first="E-De" last="Qin">E-De Qin</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Qin, Cheng Feng" sort="Qin, Cheng Feng" uniqKey="Qin C" first="Cheng-Feng" last="Qin">Cheng-Feng Qin</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">BMC Microbiology</title><idno type="eISSN">1471-2180</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Dengue virus (DENV) still poses a global public health threat, and no vaccine or antiviral therapy is currently available. Antibody plays distinct roles in controlling DENV infections. Neutralizing antibody is protective against DENV infection, whereas sub-neutralizing concentration of antibody can increase DENV infection, termed antibody-dependent enhancement (ADE). Plaque-based assay represents the most widely accepted method measuring neutralizing or enhancing antibodies.</p></sec><sec><title>Results</title><p>In this study, a novel reporter virus-based system was developed for measuring neutralization and ADE activity. A stable <italic>Renilla</italic> luciferase reporter DENV (Luc-DENV) that can produce robust luciferase signals in BHK-21 and K562 cells were used to establish the assay and validated against traditional plaque-based assay. Luciferase value analysis using various known DENV-specific monoclonal antibodies showed good repeatability and a well linear correlation with conventional plaque-based assays. The newly developed assay was finally validated with clinical samples from infected animals and individuals.</p></sec><sec><title>Conclusions</title><p>This reporter virus-based assay for neutralizing and enhancing antibody evaluation is rapid, lower cost, and high throughput, and will be helpful for laboratory detection and epidemiological investigation for DENV antibodies.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Gubler, Dj" uniqKey="Gubler D">DJ Gubler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Simmons, Cp" uniqKey="Simmons C">CP Simmons</name></author><author><name sortKey="Farrar, Jj" uniqKey="Farrar J">JJ Farrar</name></author><author><name sortKey="Nguyen, Vv" uniqKey="Nguyen V">vV Nguyen</name></author><author><name sortKey="Wills, B" uniqKey="Wills B">B Wills</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Adams, B" uniqKey="Adams 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sortKey="Deng, Yong Qiang" sort="Deng, Yong Qiang" uniqKey="Deng Y" first="Yong-Qiang" last="Deng">Yong-Qiang Deng</name><name sortKey="Jiang, Tao" sort="Jiang, Tao" uniqKey="Jiang T" first="Tao" last="Jiang">Tao Jiang</name><name sortKey="Jiang, Zhen You" sort="Jiang, Zhen You" uniqKey="Jiang Z" first="Zhen-You" last="Jiang">Zhen-You Jiang</name><name sortKey="Li, Xiao Feng" sort="Li, Xiao Feng" uniqKey="Li X" first="Xiao-Feng" last="Li">Xiao-Feng Li</name><name sortKey="Qin, Cheng Feng" sort="Qin, Cheng Feng" uniqKey="Qin C" first="Cheng-Feng" last="Qin">Cheng-Feng Qin</name><name sortKey="Qin, E De" sort="Qin, E De" uniqKey="Qin E" first="E-De" last="Qin">E-De Qin</name><name sortKey="Song, Ke Yu" sort="Song, Ke Yu" uniqKey="Song K" first="Ke-Yu" last="Song">Ke-Yu Song</name><name sortKey="Zhang, Bo" sort="Zhang, Bo" uniqKey="Zhang B" first="Bo" last="Zhang">Bo Zhang</name><name sortKey="Zhang, Fu Chun" sort="Zhang, Fu Chun" uniqKey="Zhang F" first="Fu-Chun" last="Zhang">Fu-Chun Zhang</name><name sortKey="Zhao, Hui" sort="Zhao, Hui" uniqKey="Zhao H" first="Hui" last="Zhao">Hui Zhao</name><name sortKey="Zhu, Shun Ya" sort="Zhu, Shun Ya" uniqKey="Zhu S" first="Shun-Ya" last="Zhu">Shun-Ya Zhu</name></country><country name="Singapour"><noRegion><name sortKey="Shi, Pei Yong" sort="Shi, Pei Yong" uniqKey="Shi P" first="Pei-Yong" last="Shi">Pei-Yong Shi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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